golden_gate_from_parts
shallowcom.seqbench/workbench · Verify this server
Golden Gate as the reaction runs: digest pre-domesticated part plasmids with a Type IIS enzyme and assemble them in the order their OVERHANGS dictate. The fragment released from each part is the one carrying no recognition site (the site goes out with the backbone, which is why a mis-ordered assembly is not re-cut), and the assembly order is an OUTPUT — a set whose overhangs do not close into a single cycle has no product, and the reason is the answer. Distinct from cloning_simulate's `goldengate` method, which does the other job: designing the primers that ADD the sites to BARE parts, assembled in the order you list them.
1 trials · measured 8 days ago
golden_gate_from_parts scores 100.0/100 on Vouch's measured behaviour index, from 1 real invocation trials against com.seqbench/workbench, measured 25 Aug 2026 under methodology v0.2.0. Every measured component scored 100.
Component breakdown
| Component | Weight | Value |
|---|---|---|
| Reliability | 35% | not applicable |
| Schema integrity | 25% | 100.0 |
| Failure behaviour | 15% | not applicable |
| Latency | 15% | not applicable |
| Concurrency | 10% | not applicable |
Tool details
- Transport
- remote
- Credential class
- self-provisionable
- Input schema
- not declared
- Output schema
- not declared
- Side-effect classification
- unclassified
Score history
| Day | Score | Tier | Methodology |
|---|---|---|---|
| 2026-08-25 | 100.0 | shallow | v0.2.0 |
Probe evidence
| Probe | Outcomes |
|---|---|
| schema_integrity | pass: 1 |
Raw request/response logs are not archived yet — the outcome counts above are drawn directly from every recorded trial.
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